DNA Pull-Down Service

DNA Pull-Down is an analytical technique used to study protein-DNA interactions. Generally, this experiment first requires designing and preparing specific probes (primarily labeled with desthiobiotin) targeting the regulatory region of the gene of interest. Simultaneously, nuclear extracts are prepared. The probes and nuclear extracts are incubated together, allowing DNA-binding proteins to specifically bind to the target sequences. Then, the protein-DNA complexes are purified using streptavidin magnetic beads. Finally, the obtained proteins are identified using WB validation or mass spectrometry to determine protein types.

• High Specificity: Highly specific enrichment of proteins interacting with target DNA sequences with low background interference.
• Quantitative Analysis: Enables quantitative analysis of DNA-protein interactions using fluorescent or radioactively labeled probes to measure binding strength and affinity.
• Suitable for High-Throughput Analysis: DNA Pull-Down technology allows high-throughput analysis, simultaneously studying multiple DNA-protein interactions, providing more comprehensive information and deeper insights.
• Low Sample Requirement: Requires only small amounts of sample for experiments, which is significant for research with limited or precious samples.
• Wide Applicability: Can be applied under various experimental conditions, including in vitro and in vivo studies, suitable for various biological systems and cell types. It can be used to study DNA-protein interactions in different biological processes such as gene regulation, DNA repair, chromatin structure and function. Whether studying basic biology or disease mechanisms, DNA Pull-Down technology has broad applicability.