Custom Polyclonal Antibody Service

Custom Polyclonal Antibody Service

Multiple Species
One-Stop Service
Large-Scale Production

Service Features

GeneCreate's polyclonal antibody preparation platform, with antibody technology as the core, has established a series of technical platforms and animal breeding bases. We can provide polyclonal antibody preparation services for multiple systems including rabbit, mouse, rat, sheep, and more, allowing you to freely choose according to your specific requirements!

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Service Introduction

Polyclonal antibody preparation is a technology that produces multiple different antibody clones by immunizing host animals. It is based on the characteristics of the immune system, where immunization of host animals with specific antigens induces immune cells to produce diverse antibodies to cope with the diversity and complexity of antigens. Since polyclonal antibodies can recognize multiple epitopes and cause agglutination and precipitation reactions, they are widely used in immunological diagnostics, often serving as enzyme-labeled secondary antibodies in WB experiments and coating antibodies in ELISA experiments.

Polyclonal antibodies exist in the serum of immunized animals and can be obtained by directly separating the serum. Due to their ability to recognize multiple epitopes and cause agglutination and precipitation reactions, they are widely used in immunological diagnostics, often serving as enzyme-labeled secondary antibodies in WB experiments and coating antibodies in ELISA experiments.

Service Advantages

  • Diversity: Multiple antibody clones covering multiple antigen regions, improving antigen recognition capability.
  • High Specificity: Precisely recognizes target antigens, reducing non-specific binding.
  • High Affinity: Strongly binds to target antigens, enhancing antigen-antibody interaction.
  • Flexibility: Suitable for various applications, meeting different research needs.
  • Fault Tolerance: When some antibodies fail, other clones still function, reducing failure risk.
  • Cost-Effectiveness: Relatively economical, multiple antibody clones can be obtained in one experiment.

Service Process

1
Project Analysis & Design
2
Plan Communication
3
Contract Signing
4
Project Initiation
5
Immunogen Detection
6
Animal Immunization
7
Titer Detection
8
Screening & Amplification
9
Antibody Purification
10
WB/IHC Validation
11
Results Delivery

Customer Requirements

Recombinant protein: At least 2-3 mg, protein purity >90%, concentration not less than 0.5 mg/mL, protein molecular weight >10 kDa;

Peptide: 1 mg of bare peptide, 3-4 mg of KLH/BSA/OVA or other carrier protein-conjugated peptide, bare peptide purity >90%, sequence length not less than 10 AA;

For antigen types such as virus/bacteria/strips/native protein, please consult specifically. Alternatively, you can choose to have GeneCreate prepare the antigen.

Service Description

Service Code Customer Provides Antibody Purification Method Antibody Type

Service Content

Service Cycle

(Antigen/Information)

JKR-pb001 Antigen

ProteinG/Antigen Affinity Purification

Mouse, Rat, Sheep, etc.

Polyclonal Antibodies

Antigen quality control, animal immunization,

ELISA/WB detection, blood collection,

ProteinG/antigen affinity purification, WB detection

8-10 weeks

JKR-pb002 Gene Sequence

ProteinG/Antigen Affinity Purification

Mouse, Rat, Sheep, etc.

Polyclonal Antibodies

Antigen analysis and design, full gene synthesis,

expression plasmid construction,

protein expression and purification,

animal immunization, ELISA/WB detection,

blood collection, ProteinG/antigen affinity purification,

WB detection

20-24 weeks

(Protein)
JKR-pb003 Gene Sequence

ProteinG/Antigen Affinity Purification

Mouse, Rat, Sheep, etc.

Polyclonal Antibodies

Antigen analysis and design, peptide synthesis,

carrier conjugation, conjugate quality control,

animal immunization, ELISA/WB detection,

blood collection, ProteinG/antigen affinity purification

16-20 weeks

(Peptide)

JKR-pb004

Plasmid Template

ProteinG/Antigen Affinity Purification

Mouse, Rat, Sheep, etc.

Polyclonal Antibodies

Protein expression and purification,

animal immunization, ELISA/WB detection,

blood collection, ProteinG/antigen affinity purification,

WB detection

16-20 weeks

JKR-pb005

Small Molecule Compounds

ProteinA/G Affinity Purification

Mouse, Rat, Sheep, etc.

Polyclonal Antibodies

Hapten conjugation to carrier,

small molecule conjugate quality control,

animal immunization,

ELISA detection, blood collection,

ProteinA/G affinity purification

16-20 weeks

JKR-pb006

Phosphorylation/Methylation/

Acetylation Modification Information

Antigen Affinity Purification

Modification-Specific Antibodies

Peptide synthesis (modified/non-modified peptides),

carrier conjugation, animal immunization,

ELISA/WB detection, blood collection,

antigen affinity purification

(using modified/non-modified peptides)

24-28 weeks

Case Studies

Custom Polyclonal Antibody

Ready to Start Your Co-IP Project?

Our expert team is ready to assist you with your protein interaction research. Get in touch with us for a consultation or request a quote today.

Contact Our Experts

Q&A

What is the purpose of using fusion tags for expression?