DNA Pull-Down Kit for Plant

DNA Pull-Down technology is a powerful tool for studying DNA-protein interactions in vitro. This technique involves designing specific DNA probes for target regions, which are then labeled with desthiobiotin. The labeled probes can affinity-bind with streptavidin conjugated to magnetic beads. After incubation with total protein extract, interacting proteins specifically bind to the DNA probes, forming a bead-DNA probe-protein complex. After washing away non-specifically bound proteins, the target DNA probe-protein complex is eluted and finally identified by Western Blot or Mass Spectrometry (MS). The principle is illustrated in Figure 1.1:

• High Sensitivity and Specificity: Utilizes high-affinity solid-phase carrier-streptavidin magnetic beads with an optimized experimental system, significantly reducing non-specific binding and adapting to low-abundance samples.
• Multi-dimensional Downstream Analysis Compatibility: Eluted proteins can be directly used for Mass Spectrometry (MS), Western Blot (WB), or silver staining, meeting qualitative/quantitative analysis requirements.