Animal Co-Immunoprecipitation (Co-IP) Kit

Co-Immunoprecipitation (Co-IP) is a method based on the specific interaction between antigens and antibodies, used to study protein-protein interactions. After antibodies bind to corresponding proteins in the lysate, they are incubated with Protein A/G-coupled Sepharose or Magnetic Beads. The Protein A/G bead-antibody-target protein complex is obtained through centrifugation or using a magnetic stand. Under high temperature and reducing agent conditions, the antigen and antibody dissociate, and the supernatant is collected. The supernatant contains antibodies, target proteins, and a small amount of impurity proteins, which can then be identified by Western Blot or mass spectrometry (MS). The schematic diagram is as follows:

• Precise Capture: Optimized upgrade of beads and buffers ensures efficient enrichment of target proteins and their interacting proteins, reducing non-specific binding.
• Low Background Design: Optimized elution and washing solutions reduce interference signals, improving detection rates for weak interactions, suitable for low-abundance protein research.
• Standardized Process: Detailed step-by-step manual (with operation video link), allowing beginners to get started quickly and reducing optimization time.
• Easy Operation: Saves time with objective and accurate results.