Animal Co-Immunoprecipitation (Co-IP) Kit
| Product Code | Specification |
|---|---|
| JKR23001A |
Experimental Principle
Co-Immunoprecipitation (Co-IP) is a method based on the specific interaction between antigens and antibodies, used to study protein-protein interactions. After antibodies bind to corresponding proteins in the lysate, they are incubated with Protein A/G-coupled Sepharose or Magnetic Beads. The Protein A/G bead-antibody-target protein complex is obtained through centrifugation or using a magnetic stand. Under high temperature and reducing agent conditions, the antigen and antibody dissociate, and the supernatant is collected. The supernatant contains antibodies, target proteins, and a small amount of impurity proteins, which can then be identified by Western Blot or mass spectrometry (MS). The schematic diagram is as follows:
Product Advantages
- Precise Capture: Optimized upgrade of beads and buffers ensures efficient enrichment of target proteins and their interacting proteins, reducing non-specific binding.
- Low Background Design: Optimized elution and washing solutions reduce interference signals, improving detection rates for weak interactions, suitable for low-abundance protein research.
- Standardized Process: Detailed step-by-step manual (with operation video link), allowing beginners to get started quickly and reducing optimization time.
- Easy Operation: Saves time with objective and accurate results.
Scope of Application
- Protein-protein interaction validation
- Identification of multiprotein complex members (such as transcriptional regulatory complexes, enzyme complexes, or virus-host protein complexes)
- Post-translational modification studies: Analysis of the effects of modifications such as phosphorylation and ubiquitination on protein interactions, often combined with mass spectrometry or specific antibodies
Experimental Procedure
Reagent Kit Components
Special Note 1: PBS, 6X loading buffer, magnetic stand and other reagents/consumables need to be prepared by user.
Special Note 2: 6T is for 6 single-group (1 IP group or 1 IgG group) immunoprecipitation experiments. The subsequent operation steps include 1 IgG group and 1 IP group each, consuming 2T of reagent.