Standard Primer Synthesis Service
Service Features
GeneCreate utilizes the mainstream Dr.oligo192 high-throughput primer synthesizer, professional technical personnel, and mature synthesis and purification methods to provide high-quality, diverse primer synthesis services. Multiple purification methods including RPC, PAGE, and HPLC ensure quality and timely delivery, with next-day delivery available in the Wuhan area.
Service Introduction
Standard primers are primers used in PCR reactions to guide DNA amplification, typically consisting of 18-25 bases that can specifically bind to target DNA sequences and provide the 3' end required for DNA polymerase-catalyzed synthesis of new DNA strands. Standard primers are commonly used in routine PCR reactions, including gene expression analysis, pathogen detection, and genotype identification applications. The synthesis and design of standard primers are very common and standardized, with ready-made primers typically available in primer libraries, or they can be custom synthesized as needed, offering advantages such as low cost and simple operation. Primer synthesis can be used for experiments such as PCR, sequencing, whole gene synthesis, subcloning, site-directed mutagenesis, vector construction, RNA interference, and gene recombination. Different purification methods can be selected according to different purposes.
Service Advantages
- Advanced: Utilizes the world's advanced nm-level Dr.oligo192 high-throughput DNA synthesizer from BLP Company, making primer synthesis faster, safer, and more stable;
- Fast: Can synthesize 192 primers within 30 bases in 3 hours; conventional RPC, DSL, OPC purified primers shipped within 24 hours, with next-day delivery available in the Wuhan area;
- Safe: Uses the principle of gas-phase ammoniaolysis, with improved ammoniaolysis methods that enable faster and more complete primer ammoniaolysis within 90 minutes, avoiding unnecessary mutations caused by other reagent ammoniaolysis;
- High Quality: Multiple purification methods, multiple quality testing methods (HPLC, MS), comprehensively ensuring our shipping quality.
Service Process
note:standard primer synthesis service.
Customer Requirements
Primer synthesis order form: Need to fill in detailed customer information, sequence, number of bases, purification method, etc.
Service Description
| Purification Method | Primer Length |
Yield (OD) |
Delivery Time |
| RPC Purification | 8-15nt |
1-2 |
1 business day |
|
2-4 |
1 business day | ||
| 15-59nt |
1-4 |
1 business day | |
|
5-10 |
1 business day | ||
|
10-15 |
1 business day | ||
|
15-20 |
1 business day | ||
|
>20 |
1 business day | ||
| 60-120nt |
1-2 |
3 business days | |
|
2-5 |
3 business days | ||
|
>5 |
3 business days | ||
| PAGE Purification | 15-59nt |
1-2 |
3 business days |
|
3-4 |
3 business days | ||
|
5-8 |
3 business days | ||
|
>8 |
3 business days | ||
| 60-80nt |
1-2 |
3 business days | |
|
3-4 |
3 business days | ||
|
5-8 |
3 business days | ||
|
>8 |
3 business days | ||
| 80-100nt |
3 |
3 business days | |
|
3 |
3 business days | ||
|
3 |
3 business days | ||
|
>8 |
3 business days | ||
| 100-120nt |
1 |
3 business days | |
|
2 |
3 business days | ||
| HPLC Purification | 8-15nt |
1-2 |
3-5 business days |
|
3-4 |
3-5 business days | ||
| 15-59nt |
1-4 |
3-5 business days | |
|
5-8 |
3-5 business days |
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Contact Our ExpertsQ&A
Why must a sequencing primer bind specifically to the DNA template?
Why might a dissolved primer work initially but perform poorly after some time?
How should synthesized DNA be stored?
When analyzing synthetic Oligo DNA products using 3% Agarose gel electrophoresis, many bands are observed, Why?