DNA Pull-Down Kit (Animal)

DNA Pull-Down technology is a powerful tool for studying protein-DNA interactions in vitro. This technique designs specific DNA probes for target regions and labels them with desthiobiotin. The labeled probes can affinity-bind with streptavidin conjugated to magnetic beads, then incubate with total protein extract. Interacting proteins can specifically bind to the DNA probe, forming a magnetic bead-DNA probe-protein complex. After washing away non-specifically bound proteins, the target DNA probe-protein complex is obtained through elution, and finally, protein types are identified by Western Blot or Mass Spectrometry (MS). The principle is illustrated in Figure 1.1:

• High Sensitivity and Specificity: Utilizes high-affinity solid-phase carriers (streptavidin magnetic beads) with an optimized experimental system that significantly reduces non-specific binding and is suitable for low-abundance samples.
• Multi-dimensional Downstream Analysis Compatibility: Eluted proteins can be directly used for Mass Spectrometry (MS), Western Blot (WB), or silver staining, meeting qualitative/quantitative analysis requirements.