Monoclonal Antibody Customization Service
Service Features
GeneCreate's antibody platform has extensive and unique experience in mouse monoclonal antibody development, with mature technical routes and stable, reliable, and highly sensitive products. In recent years, the platform has continuously optimized the monoclonal antibody experimental system, making significant improvements from immunization and hybridoma cell fusion to positive hybridoma cell screening, greatly increasing fusion rates and positive rates, enabling the preparation of the highest quality monoclonal antibodies in the shortest time.
Service Introduction
Compared to polyclonal antibodies, monoclonal antibodies target a single antigenic epitope, offering higher specificity and sensitivity. Using monoclonal antibodies can eliminate serological cross-reactions between different cells and microbial species or strains, greatly improving diagnostic specificity and sensitivity. Meanwhile, monoclonal antibodies play a crucial role in researching cell surface markers, purifying soluble antigens, further studying antibody structure and function, and antibody drug development.
GeneCreate provides customized antibody services for both monoclonal and polyclonal antibodies. We have delivered thousands of antibody projects, covering small molecule antibodies, plant antibodies, research and diagnostic antibodies, with validation types including IHC, WB, IP, ELISA, ChIP, FC, etc. For customized monoclonal antibodies, GeneCreate offers a complete solution from antigen design, animal immunization, antibody purification to antibody validation. Additionally, GeneCreate has antibody discovery platforms including hybridoma and phage antibody libraries, allowing selection of the most suitable platform based on your antibody application and validation requirements.
Service Advantages
- One-stop solution: Provides comprehensive antibody services from gene synthesis, antigen expression to antibody preparation
- Large-scale production: Offers the most professional monoclonal antibody large-scale production services to meet diagnostic-level mass production needs
- Strict quality control: Provides application-oriented antibody customization for basic experiments like ELISA, WB, IHC, as well as clinical diagnostics including chemiluminescence, colloidal gold, and latex-enhanced immunoturbidimetry
Service Process
Our monoclonal antibody customization service follows a rigorous 11-step process to ensure accurate and reliable results. Each step is carefully monitored and quality-controlled to maintain the highest standards in antibody production.
Customer Requirements
1. Recombinant protein: At least 2-3 mg, protein purity >90%, concentration not less than 0.5 mg/mL, protein molecular weight >10kDa
2. Peptide: 1 mg naked peptide, 3-4 mg conjugated to KLH/BSA/OVA or other carrier proteins, naked peptide purity >90%, sequence length not less than 10 AA
3. For other antigen types such as viruses/bacteria/strips/natural proteins, please consult specifically. Alternatively, you can choose to have GeneCreate prepare the antigen.
Service Description
|
Service Process |
Deliverables | Service Cycle |
|
Antigen Analysis & Design |
Immunogen sequence | 1 working day |
|
Antigen Preparation |
Recombinant protein preparation: Expression clone sequencing report | 4-7 weeks |
|
Peptide synthesis & conjugation: MS and HPLC quality control report |
3-4 weeks | |
| Animal Immunization |
50 pre-immune serum |
6-8 weeks |
| Titer Detection |
Antiserum ELISA and WB detection results |
1-2 working days |
| Fusion |
/ |
1 working day |
| Subcloning Screening |
Intermediate subclone detection results according to customer requirements |
4-8 weeks |
|
Strain Cloning Isotype Identification & Antibody Purification Validation |
Screen <20 high-affinity hybridoma cell culture supernatants sent to customers for validation to select monoclonal antibodies with more application requirements. Communication confirmation followed by antibody purification and final validation. |
2-3 weeks |
|
Final Report & Delivery |
Pre-delivery final report containing all raw data, images, and quality control reports during the customization process. After mutual confirmation, deliver all contents as agreed in the contract. |
3-5 working days |
Case Studies
Ready to Start Your Co-IP Project?
Our expert team is ready to assist you with your protein interaction research. Get in touch with us for a consultation or request a quote today.
Contact Our ExpertsQ&A
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How to select an appropriate host cell?
How can soluble protein expression be improved?
What causes proteins expressed in the bacterial cytoplasm to form inclusion bodies?