RNA Pull-Down Kit
| Product Code | Specification |
|---|---|
| JKR23004 |
Experimental Principle
RNA pull-down is a technique used to study RNA interactions with proteins or other RNA molecules within cells. The RNA is first labeled (e.g., with biotin) and then incubated with cell lysates to form RNA-RNA/protein complexes. These complexes are then isolated and analyzed to identify interacting molecules.
After elution, the complexes can be analyzed by various methods: quantitative PCR (RNA pull-down-qPCR) or high-throughput sequencing (RNA pull-down-seq) to identify RNA-RNA interactions, and Western blot (pull-down-WB) or mass spectrometry (pull-down-MS) to detect RNA-protein interactions.
Product Advantages
- High Specificity: Magnetic beads provide low background noise; non-specific RNA or mutant RNA does not significantly affect the enrichment of specific RNA-binding proteins.
- Ultra-High Sensitivity: Capable of detecting low-abundance RNA and weak interaction proteins, suitable for precious samples (such as clinical biopsies and single-cell samples).
- Reliable Results: Optimized protocol ensures consistent and reproducible outcomes.
- Comprehensive Support: Includes detailed manual and technical support for successful experimentation.
Scope of Application
- RNA-Protein Interaction Studies:
- Discovery of novel RNA-binding proteins: Capture proteins interacting with specific RNAs (mRNA, lncRNA, circRNA, etc.) to reveal unknown regulatory networks.
- Validation of known interactions: Precisely detect physical binding between target RNA and candidate proteins (such as transcription factors, enzymes) to support mechanistic studies.
- Functional analysis of non-coding RNAs and biomarker discovery.
Experimental Procedure
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Reagent Kit Components
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