Double-Luciferase Reporter Assay Kit

Firefly luciferase and Renilla luciferase catalyze the oxidation of luciferin or coelenterazine to form oxyluciferin or coelenteramide, respectively, producing bioluminescence in the process. The Double-Luciferase Reporter Assay Kit first detects the activity of the firefly luciferase reporter gene using luciferin as a substrate, then quenches this reaction while detecting the activity of the Renilla luciferase reporter gene using coelenterazine as a substrate. This system offers rapid detection, high sensitivity, broad detection range, and no interference from endogenous cellular activities.

• This product demonstrates significantly higher detection sensitivity compared to similar products on the market, with stable chemiluminescent signals.
• The luminescence intensity is superior to other brands.
• Optimal firefly luciferase quenching effect, far exceeding other market brands, with minimal fluorescence residue that has almost no impact on Renilla luciferase reluminescence, improving test result accuracy and data reproducibility.
• Excellent stability, simple operation, stable readings, and fast detection speed.