Electrophoretic Mobility Shift Assay (EMSA) Service

Electrophoretic Mobility Shift Assay (EMSA) is a technique used to detect protein-nucleic acid interactions, providing qualitative and quantitative analysis. It has been widely used to study DNA-binding proteins and their interactions with specific DNA sequences, making it a classical method for transcription factor research.

Validation EMSA

Used to verify whether the probe contains binding sites for proteins. By mutating the binding sites and using the mutant probe as a control, if the wild-type probe shows a shifted band while the mutant probe does not, it indicates that the probe contains protein-binding sites.

Competition EMSA

The probe sequence remains unchanged, and unlabeled probes are called cold probes. Cold probes compete with labeled probes for protein binding, but cold probes do not produce visible shifted bands. Using cold probes as controls, if the labeled probe shows a shifted band while the cold probe's band weakens or disappears, it eliminates false-positive interference and increases experimental accuracy.

Supershift EMSA

Uses specific antibodies against the target protein. The protein-probe complex is recognized and bound by the antibody, forming a ternary complex that migrates faster in gel electrophoresis, appearing as a supershifted band above the protein-probe complex, confirming the specific binding between the probe and the target protein.

• Direct detection of protein-nucleic acid interactions, providing qualitative and quantitative information.
• Can be used to study various types of interactions, such as specific binding, non-specific binding, and sequence specificity.
• Simple technology with relatively easy experimental operation.
• Can be adapted to different experimental needs using various labeling methods and detection techniques.