Silver Stain Kit
| Product Code | Specification |
|---|---|
| JKR23005 |
Experimental Principle
Silver staining detects proteins in polyacrylamide gels through a chemical process where silver ions are reduced to metallic silver under alkaline pH conditions. The metallic silver precipitates on protein surfaces, creating visible bands. This method offers significantly higher sensitivity compared to conventional staining techniques, enabling detection of low-abundance proteins.
Experimental Procedure
Silver staining is used to detect proteins in polyacrylamide gels, based on the principle
that silver ions are reduced to metallic silver in an alkaline pH environment,
precipitatingontheproteinsurfacetoproducecolor.
Reagent Kit Components
Important Notes:
- Due to the high sensitivity of silver staining, use high-purity water and ensure all equipment is thoroughly clean. Glassware is recommended.
- Always wear gloves during operation to avoid direct skin contact with gels.
- Customer to provide: ethanol, acetic acid, and deionized water.
- Silver solution is corrosive. Handle with care using appropriate personal protective equipment. Avoid environmental release as it is toxic to aquatic organisms.
- This product is for professional research use only. Not for diagnostic, therapeutic, food, or pharmaceutical applications. Do not store in residential areas.
- For your safety, wear lab coats and disposable gloves during operation.