Modified/Labeled Primers Service
Service Features
GeneCreate possesses strong primer synthesis capabilities and can quickly synthesize high-quality ordinary primers for you. We also provide various types of primer modification and labeling services.
Service Introduction
Modified/labeled primers refer to primers that have been enhanced with specific chemical modifications or fluorescent labels based on ordinary primers to improve PCR reaction specificity and detection sensitivity. Common primer modification forms include phosphorylation, biotin, digoxigenin, internal amino modification, 5' amino modification, 3' amino modification, etc., which can be used for various non-radioactive immunoassays and hybridization reactions. These modifications/labels enable primers to bind more tightly to target DNA sequences, increasing the specificity and selectivity of PCR reactions, while also making amplification products easier to detect and analyze in subsequent detection processes. Modified/labeled primers are widely used in pathogen detection, genotype identification, gene expression analysis, and other fields, providing scientists with more precise and highly sensitive molecular biology tools.
Service Advantages
- Rich variety of primer modification/labeling types, with average mutation rate below 1/1000 verified by large-scale sequencing
- Strict implementation of ISO 9001 and ISO 13485 unified quality control standards, strong product traceability, and complete batch records
- Multiple purification methods available, customized specifications, shipping forms, and special primer synthesis according to needs
- Convenient and complete online primer synthesis ordering system, experienced primer synthesis expert team and technical support
Service Process
note:modified oligo synthesis
Customer Requirements
Primer Synthesis Order Form
Detailed information including customer information, sequence, base count, purification method, etc., must be provided.
Service Description
|
Category |
Modification Type | Purification Method |
| Rare Bases |
dI |
PAGE |
|
dU |
PAGE | |
| Thio Modification |
Phosphorthioate |
PAGE |
| Phosphate Group |
5' PO4 |
HPLC |
| Amino |
5' NH2 C6 |
HPLC |
|
3' NH2 C7 |
HPLC | |
| Sulfhydryl |
3' SH C6 |
HPLC |
|
5' SH C6 |
HPLC | |
| Biotin |
5' Biotin |
HPLC |
|
3' Biotin |
HPLC | |
| Fluorophores |
5' Cy3 |
HPLC |
|
5' Cy5 |
HPLC | |
|
5' FAM |
HPLC | |
|
3' FAM |
HPLC | |
|
5' HEX |
HPLC | |
|
5' TET |
HPLC | |
|
5' Rox |
HPLC | |
|
5' TAMRA |
HPLC | |
|
3' TAMRA |
HPLC | |
|
3' BHQ 1 |
HPLC | |
|
3' BHQ 2 |
HPLC | |
|
3' Dabcyl |
HPLC | |
| Dual Labeling |
5' FAM-3' TAMRA |
HPLC |
|
5' FAM-3' BHQ1 |
HPLC | |
|
5' FAM-3' BHQ2 |
HPLC | |
|
5' FAM-3' Dabcyl |
HPLC | |
|
5' HEX-3' TAMRA |
HPLC | |
|
5' HEX-3' BHQ1 |
HPLC | |
|
5' HEX-3' BHQ2 |
HPLC | |
|
5' HEX-3' Dabcyl |
HPLC | |
|
5' TET-3' TAMRA |
HPLC | |
|
5' TET-3' BHQ1 |
HPLC | |
|
5' TET-3' BHQ2 |
HPLC | |
|
5' TET-3' Dabcyl |
HPLC | |
|
5' Cy3-3' BHQ2 |
HPLC | |
|
5' Cy5-3' BHQ2 |
HPLC | |
| Others |
Please inquire |
|
Case Studies
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Contact Our ExpertsQ&A
What are the requirements for sequencing primers?
What is the criterion for judging the quality of a primer?
What could be the reason if annealed primer fragments cannot be ligated into a vector?