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For protein expression, if providing a plasmid (cloning plasmid or expression plasmid), what information is needed?
1. Sequencing results, including the sequence and chromatogram.
2. Plasmid vector name.
3. Recombinant cloning plasmid (10 µl) or expression plasmid (20 µl) with concentration >100 ng/µl.
4. Plasmid antibiotic resistance.
5. Primers used for cloning and sequencing.
What are the main steps in the E. coli expression system?
(1) Obtaining the target gene.
(2) Plasmid construction.
(3) Strain screening.
(4) Expression condition optimization.
(5) Protein purification.