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How does the relative activity of Renilla Luciferase compare to Firefly Luciferase?
Firefly luciferase acts on luciferin in the presence of oxygen, magnesium, and ATP, while luciferase from the sea pansy (Renilla reniformis) acts on coelenterazine in the presence of oxygen. Dual-reporter assays combine Firefly luciferase and Renilla luciferase tests.