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When analyzing synthetic Oligo DNA products using 3% Agarose gel electrophoresis, many bands are observed, Why?
Denaturing PAGE electrophoresis must be used for Oligo DNA. Oligo DNA is single-stranded and prone to forming complex secondary structures. Therefore, during Agarose gel electrophoresis, multiple bands often appear (and Agarose gel electrophoresis cannot be used for quantification).