Genetic Research Q&A Platform
Get expert answers to your genetic research questions. Connect with scientists and researchers worldwide.
During PAGE electrophoresis, why don't Oligo DNAs of exactly the same length migrate to the same position?
The composition of A, G, C, T differs, leading to different electrophoretic mobilities.The secondary structures of the DNA differ, leading to different electrophoretic mobilities.This phenomenon is more likely to occur with shorter Oligo DNAs; the differences between long-chain Oligo DNAs are smaller.