Why provide fresh bacterial culture? How to provide fresh bacterial culture?

Firstly, fresh bacterial culture is easy to grow, allowing for more DNA yield, while also maximizing the purity of the strain. If providing fresh culture, seal the cap with parafilm to prevent leakage. Alternatively, pellet 4–5 mL of the cultured bacteria, pour off the supernatant for easier shipping. When mailing, it's best to use a box to prevent the tube from being crushed during transit.