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What are the requirements for plasmid DNA used for sequencing?
General requirements for sequencing plasmid DNA:
(1) High DNA purity (A260/A280 ratio 1.6–2.0). It must not contain mixed templates, RNA, genomic DNA, or proteins.
(2) Dissolved in ddH₂O. The solution must not contain impurities such as salts or chelators like EDTA, as these will interfere with the sequencing reaction.