Genetic Research Q&A Platform
Get expert answers to your genetic research questions. Connect with scientists and researchers worldwide.
For exosome-related proteins like CD81, CD9, CD63, and calnexin, can they not be stored after boiling? I heard that because these are membrane proteins, they cannot be boiled? Other markers like HSP70 and TSG101 worked fine. Is this true?
Membrane proteins are generally not recommended for boiling. Usually, after boiling the sample, the bands appear very smeared and cannot be recognized well by the antibody. This might be because some antibodies recognize conformational (3D) epitopes rather than linear epitopes.