For detecting exosomal protein markers by flow cytometry, are beads necessary? Are there specific steps?

Flow cytometry can detect exosomal marker proteins. Specific steps are as follows:

(1) Process the sample and extract exosomes.

(2) Antibody incubation: Resuspend the extracted exosomes in PBS containing 2% BSA. Perform primary and secondary antibody incubations sequentially to obtain the CD63-labeled exosome detection solution.

(3) Set flow cytometer parameters: Run the flow cytometer normally and set the parameters.

(4) Detection: Resuspend the CD63-labeled exosome detection solution in PBS and load it for detection.

(5) Use flow cytometry analysis software to analyze the data and obtain quantitative detection results for exosomes.