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Latest Questions
What is the maximum length of DNA and RNA that your company can synthesize?
DNA synthesis length: 6–130 bases, RNA synthesis length: 8–35 bases.
When synthesizing long DNA sequences, it is difficult to ensure that every molecule in the product is completely correct due to limitations in synthesis and purification methods. Additionally, it should be noted that if the sequence to be synthesized is particularly challenging (e.g., containing consecutive "G"s, etc.), the synthesis yield may be relatively low, and we may charge an additional fee based on the specific situation. Sometimes, we may be unable to synthesize the ordered sequence, in which case the company reserves the right to decline the order.
What could be the reason if annealed primer fragments cannot be ligated into a vector?
If the primers in the ligation reaction lack a 5' phosphate group, the ligation efficiency is relatively low, but it may not completely fail.
If the phosphorylated product still cannot be ligated into the vector, it is necessary to check the vector digestion efficiency and review the primer design. If no issues are found, consider optimizing the primer annealing conditions and the ligation reaction system.