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Latest Questions
Do general synthetic Oligo DNAs have phosphate groups at the 5' and 3' ends?
No, Both the 5' and 3' ends are -OH groups. If phosphate groups are required, please specify this when ordering. In this case, a phosphorylation (PO4 modification) fee will be charged.
During PAGE electrophoresis, why don't Oligo DNAs of exactly the same length migrate to the same position?
The composition of A, G, C, T differs, leading to different electrophoretic mobilities.The secondary structures of the DNA differ, leading to different electrophoretic mobilities.This phenomenon is more likely to occur with shorter Oligo DNAs; the differences between long-chain Oligo DNAs are smaller.