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For exosome-related proteins like CD81, CD9, CD63, and calnexin, can they not be stored after boiling? I heard that because these are membrane proteins, they cannot be boiled? Other markers like HSP70 and TSG101 worked fine. Is this true?
Membrane proteins are generally not recommended for boiling. Usually, after boiling the sample, the bands appear very smeared and cannot be recognized well by the antibody. This might be because some antibodies recognize conformational (3D) epitopes rather than linear epitopes.
I plan to study exosomal lncRNA. The lncRNA expression is lower in tumor tissue compared to normal tissue, but higher in exosomes. Is there a theory to explain this?
Exosomes are extracellular nano-sized vesicles of endocytic origin. Circulating exosomes in body fluids can interact with recipient cells by carrying various functional molecules like lncRNA, miRNA, proteins, etc., mediating intercellular communication and influencing tumor occurrence and development. LncRNAs detected in exosomes are termed exosome-derived lncRNAs. Studies have found that exosomes can transfer tumor-associated lncRNAs to tissue cells, affecting tumor biological processes, playing important roles in tumor proliferation, metastasis, invasion, drug resistance, immune regulation, and angiogenesis. Furthermore, tumor-derived exosomes have the ability to precondition distant or specific sites. Transferring lncRNAs to recipient cells at these sites can create a pre-metastatic niche suitable for tumor cell growth. Therefore, changes in the tumor microenvironment may lead to altered lncRNA content within the tumor tissue itself.