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During PAGE electrophoresis, why don't Oligo DNAs of exactly the same length migrate to the same position?
The composition of A, G, C, T differs, leading to different electrophoretic mobilities.The secondary structures of the DNA differ, leading to different electrophoretic mobilities.This phenomenon is more likely to occur with shorter Oligo DNAs; the differences between long-chain Oligo DNAs are smaller.
How does the relative activity of Renilla Luciferase compare to Firefly Luciferase?
Firefly luciferase acts on luciferin in the presence of oxygen, magnesium, and ATP, while luciferase from the sea pansy (Renilla reniformis) acts on coelenterazine in the presence of oxygen. Dual-reporter assays combine Firefly luciferase and Renilla luciferase tests.